Potassium Stimulation of Corn Root Plasmalemma ATPase
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چکیده
منابع مشابه
Potassium Stimulation of Corn Root Plasmalemma ATPase'
The stimulation by K+ of the initial rate of Hf-pumping by ATPase was studied in native plasmalemma (Zea mays L. var Mona) vesicles and in reconstituted vesicles with enzyme purified on a glycerol gradient. In reconstituted vesicles, a very high H+pumping rate (200,000% quenching per minute per milligram protein) was obtained with 9-amino-6-chloro-2-methoxyacridine provided that the pump was sh...
متن کاملHistochemical Evidence for the Occurrence of Oligomycin-sensitive Plasmalemma ATPase in Corn Roots.
A cytochemical study has been made on the localization of ATPase activity in corn (Zea mays L.) roots. Light microscopy shows washing for 4 hours to increase the general ATPase activity in the peripheral layers of the root cortex; oligomycin and N,N-dicyclohexylcarbodiimide inhibit this activity, oligomycin being more effective. Ultrastructural studies of ATPase location show oligomycin treatme...
متن کاملIsolation of NADH Oxidation System from the Plasmalemma of Corn Root Protoplasts.
A plasmalemma-bound NADH oxidation system (Lin 1982 Proc Natl Acad Sci USA 79: 3773-3776) in corn root protoplasts was isolated by a mild treatment of intact protoplasts with trypsin. The majority of NADH stimulated O(2) consumption activity of the protoplasts could be recovered in the supernatant isolated from the intact protoplasts which have been treated with trypsin. The activation energy o...
متن کاملFurther Characterization on the Transport Property of Plasmalemma NADH Oxidation System in Isolated Corn Root Protoplasts.
Recent experiments show that exogenous NADH increases the O(2) consumption and uptake of inorganic ions into isolated corn (Zea mays L. Pioneer Hybrid 3320) root protoplasts (Lin 1982, Proc Natl Acad Sci USA 79: 3773-3776). A mild treatment of protoplasts with trypsin released most of the NADH oxidation system from the plasmalemma (Lin 1982 Plant Physiol 70: 326-328). Further studies on this sy...
متن کاملGlucagon stimulation of mitochondrial ATPase and potassium ion transport.
The hormone glucagon stimulates hepatic gluconeogenesis. Studies on the mechanism of this stimulation have centered upon those changes manifested in the hepatic mitochondria after the hormonal treatment [l-5] . Recently, acute glucagon treatment of fed, intact rats has been shown to produce a stimulation of respiration with a number of substrates in isolated, hepatic mitochondria [5]. Rates of ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1990
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.93.3.1175